The process is quite large to be explained in details.. but everything is covered in Admin guide..
I will just outline the overall process briefly..Any specific please ask the specific question separately..
Create a group and add all the user to the group .. or you can have a Windows AD group created with all the 100 users and then import the group from CMC > Authentication
Application access:
Now go to the CMC> Application > Webi and grant all the access you want in webi
Same for Deski
Content Access:
Then the next step is to grant access to the group for the content .
Go to CMC > Folder > right click and select Assign secirity > and grant access to the group
You will need to make sure granting edit access and add object access for the folder
Data Access :
Go to CMC > Connection > grant access to retrieve data
Also grant the access similarly to the universe